pld2 ko b16f10 cell lines mouse b16f10 melanoma cells  (ATCC)

Journal: Bioactive Materials

Article Title: “Artificial platelet injection system”: a plug-and-play platelet-based lysosome-targeting chimera for targeted protein degradation

doi: 10.1016/j.bioactmat.2026.01.011

Figure Lengend Snippet: │ PD-L1 degradation efficacy in vitro and in vivo by PLT-TACs in B16F10 cells. A , Western blot analysis of PD-L1 in IFN-γ pre-treated B16F10 cells after treated with saline, PLT-anti-PD-L1, PLT-TAC l , PLT-TAC m or PLT-TAC h (at an equivalent amount of 5 μM) for 36 h (n = 3 biologically independent samples). B , Mean fluorescence intensity of levels of surface PD-L1 in IFN-γ pre-treated B16F10 after treated with saline, PLT-anti-PD-L1, PLT-TAC l , PLT-TAC m or PLT-TAC h , measured by FCM (at an equivalent amount of 5 μM) for 36 h (n = 3 biologically independent samples). C , Schematic schedule of the treatment and evaluation in C57BL/6 mice with B16F10 homografting tumor model. D , Tumor growth curves, E , tumor weights, F , images of dissected tumors, G , survival curves from treatment groups (saline, PLT-anti-PD-L1, PLT-TAC l , PLT-TAC m or PLT-TAC h ) at a dosage of 5 mg/kg (n = 6 biologically independent samples). H , Immunohistochemical images of PD-L1 in dissected B16F10 tumors after treatment. I , Mean fluorescence intensity of PD-L1 in CD45-negative tumor cells from dissected tumors after treatment (n = 4 biologically independent samples). Data are presented as mean ± SD. p values were determined by one-way ANOVA test. ns, no significance; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Breast tumor cell line 4T1 and melanoma tumor cell line B16F10 were purchased from the American Type Culture Collection (ATCC), and cultured in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin.

Techniques: In Vitro, In Vivo, Western Blot, Saline, Fluorescence, Immunohistochemical staining

Journal: bioRxiv

Article Title: Cell Type–Dependent Uptake of Extracellular Vesicles Independent of Cellular Origin

doi: 10.64898/2026.05.19.726167

Figure Lengend Snippet: Uptake of HEK293T-derived EVs by murine melanoma cell lines. (A) Quantification of EV uptake following 2 h incubation of HEK293T-derived mNG-labeled EVs with B16F10 and Yummer melanoma cells at EV concentrations of 1 × 10 9 , 5 × 10 9 , and 1 × 10 10 particles. (B) Representative flow cytometry plots corresponding to panel A. (C) Quantification of EV uptake following 4 h incubation at the indicated concentrations. (D) Representative flow cytometry plots corresponding to panel C. Data are presented as mean ± SD (n = 3 independent experiments). Statistical analysis was performed using two-way ANOVA. Statistical significance is indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Human embryonic kidney cells (HEK293T), SKBR-3 human breast cancer cells, pancreatic ductal adenocarcinoma (PDAK) cells, HepG2 human hepatic cancer cells, Huh7 human hepatocellular carcinoma cells, Caco-2 human colorectal adenocarcinoma cells, and the murine melanoma cell lines B16F10 and Yummer (all obtained from ATCC) were cultured in DMEM supplemented with 10% FBS and 1% antibiotic–antimycotic solution.

Techniques: Derivative Assay, Incubation, Labeling, Flow Cytometry

Journal: bioRxiv

Article Title: Cell Type–Dependent Uptake of Extracellular Vesicles Independent of Cellular Origin

doi: 10.64898/2026.05.19.726167

Figure Lengend Snippet: Uptake of Yummer-derived EVs by murine melanoma cell lines. (A) Quantification of EV uptake following 2 h incubation of Yummer-derived mNG-labeled EVs with B16F10 and Yummer cells at EV concentrations of 1 × 10 9 and 1 × 10 10 particles. (B) Representative flow cytometry plots corresponding to panel A. (C) Quantification of EV uptake following 4 h incubation at the indicated concentrations. (D) Representative flow cytometry plots corresponding to panel C. Data are presented as mean ± SD (n = 3 independent experiments). Statistical analysis was performed using two-way ANOVA. Statistical significance is indicated as follows: ****p < 0.0001.

Article Snippet: Human embryonic kidney cells (HEK293T), SKBR-3 human breast cancer cells, pancreatic ductal adenocarcinoma (PDAK) cells, HepG2 human hepatic cancer cells, Huh7 human hepatocellular carcinoma cells, Caco-2 human colorectal adenocarcinoma cells, and the murine melanoma cell lines B16F10 and Yummer (all obtained from ATCC) were cultured in DMEM supplemented with 10% FBS and 1% antibiotic–antimycotic solution.

Techniques: Derivative Assay, Incubation, Labeling, Flow Cytometry

Journal: bioRxiv

Article Title: ME1 Programs Latent Effector Capacity and Grounds a Mathematical Model of Reversible T Cell Exhaustion

doi: 10.64898/2026.05.05.722814

Figure Lengend Snippet: (A) Schematic illustrating the generation of CD8-specific ME1 transgenic (ME1 Tg) mice. (B) Representative flow cytometry showing tdTomato expression as a surrogate for ME1 overexpression in CD8 + tumor-infiltrating lymphocytes (TILs). (C–D) Average growth curves of B16F10 tumors in control and CD8-ME1 Tg mice treated with PBS (C) or combined anti-PD-1/anti-PD-L1 antibodies (100 μg each per dose) administered every other day starting on day 6 (arrow) after tumor implantation. Tumor growth was analyzed by two-way ANOVA (D, n = 6, **P < 0.01). (E) Tumor sizes were measured at endpoint. Data were analyzed using an unpaired two-tailed t test (n = 4–8, ***P < 0.001). One of two independent experiments is shown. (F) Flow cytometric analysis of granzyme B (GZMB) protein expression in CD8 + TILs cells from B16F10 tumors in ME1 Tg and control mice on day 12. Data were analyzed using an unpaired two-tailed Student’s t test ( * P< 0.05; n = 5 mice per group).

Article Snippet: B16F10 murine melanoma cell line was purchased from ATCC (CRL-6475) and cultured using DMEM complete medium.

Techniques: Transgenic Assay, Flow Cytometry, Expressing, Over Expression, Control, Tumor Implantation, Two Tailed Test